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Biocatalysis and Biotransformation

Biocatalysis and Biotransformation

Biocatalysis is the utilisation of isolated enzymes or enzymes that are still present inside living cells to carry out chemical transformations of organic molecules. It is the metabolic change of compounds in order to produce new chemicals for industrial use. Enzymes play a critical role in hundreds of events, including the generation of alcohols from fermentation and the manufacture of cheese from the breakdown of milk proteins. Recent breakthroughs in scientific research have aided in the understanding of enzyme structure and functional activities, resulting in increased stability, activity, sustainability, and substrate specificity. Currently, there are hundreds of different biocatalytic processes in use in the pharmaceutical, chemical, food, and agricultural industries. In many aspects, biocatalytic processes are similar to conventional processes, and the most important considerations are reaction kinetics and stability for both single and multistep processes.

Biotransformation is the process of converting hydrophobic molecules into hydrophilic molecules in order to enable removal from the body. Typically, this technique produces compounds with minimal or no toxicological consequences. Through a process known as bioactivation, biotransformations can sometimes produce harmful metabolites. Biotransformations including enzymes and entire microbial cells have been used to make meals and beverages for generations. However, it was only in the last century that approaches for understanding and improving biocatalyst stability and productivity were discovered.

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